Beamline 8.3.1

ALS

Technical specifications

  • General:

    • Applications: Protein crystallography (PX), Small-molecule crystallography, X-ray diffraction (XRD)
    • Primary detector: Pilatus3 S 6M with up to 25Hz data collection
    • Secondary detector: Hitachi Vortex EX energy-resolving for MAD and excitation scans

  • Beam characteristics:

    • Min-Max energy (EV): 5000-17000
    • Wavelength range (Å): 0.73-0.25
    • Flux/brightness: 1.0 x 10e12 photons/s measured at 3 x 0.35 milliradian divergence at 11,111 eV with ALS at 500 mA
    • Flux/brightness: 1.0 x 10e12 photons/s measured at 3 x 0.35 milliradian divergence at 11,111 eV with ALS at 500 mA
    • Resolving power: Water-cooled flat double Si(111) Khozu monochromator: E/ΔE = 7,000 measured with Ge(11,1,1water-cooled silicon bent into parabola 200 nanoradian slope ) analyzer crystal
      • Primary Mirror: flat internallyerror
      • Secondary Mirror: uncooled cyllindrical silicon bent into torroid
      • 2:1 demagnification
      • Both mirrors coated with Pt/Rh
    • Spot size: 0.08 x 0.06 mm (v x h)

  • Additional features:

    • Sample temperature adjustable from 100-300K
    • “Cool Hand Luke” Robot accepts all major pin types (Hampton, Yale, SPINE, etc.) and lengths (10-24 mm) and supports “delayed data collection mode” where data collection runs are deferred to the end of the shift. This robot does not work directly with cassettes because hand-mounting of samples in the mini-hutch is faster than any robot.
    • In-Situ Tray Goniometer: Goniometer is fast air-bearing spindle capable of “true” MAD with round-robin inverse beam and wavelength changes
      • All SBS compliant crystallization trays supported. Thin-walled in-situ ready trays such as in-situ-1 highly recommended
      • Ambient temperature only currently supported